Estudo da interação entre anticorpos monoclonais anti-Streptococcus mutans e antígenos de estreptococos orais

Authors

  • Fujiko Y. Kretchetoff
  • Antonio Carlos V. Canettieri
  • Daniela Moreira
  • Fabio C. Fujarra
  • Teresa G. Oliveira
  • Carmelinda S. Unterkircher

DOI:

https://doi.org/10.14295/bds.2006.v9i1.276

Abstract

Nesse trabalho estudou-se a reatividade de dois anticorpos monoclonais (Acmo) anti-Streptococcus mutans (24A e 56G) desenvolvidos no Laboratório de Imunologia da Faculdade de Odontologia de São José dos Campos-UNESP, frente às cepas de estreptococos orais (S. salivarius, S. mutans e S. pyogenes) pelo ELISA e Western blot. Estes anticorpos eram da classe IgG2b e foram purificados em proteína A. O Acmo 56G mostrou ser o mais reativo com S. mutans. Testado no Western Blot (WB) com o antígeno citoplasmático (AgC) de S.mutans, 56G marcou uma única banda de 53kDa. Com o antígeno de superfície (AgSu) e com células inteiras dos microrganismos estudados, 56G reagiu com componentes de pesos moleculares mais elevados como 116 e 155 kDa. Foi detectada reatividade cruzada do Acmo 56G, no WB, com outros estreptococos orais (S. pyogenes, S. salivarius, S. sobrinus), constatando o compartilhamento de antígenos entre as espécies testadas, o que pode ser um fator importante no desenvolvimento de doenças, como a febre reumática.

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Published

2010-08-12

How to Cite

1.
Kretchetoff FY, Canettieri ACV, Moreira D, Fujarra FC, Oliveira TG, Unterkircher CS. Estudo da interação entre anticorpos monoclonais anti-Streptococcus mutans e antígenos de estreptococos orais. BDS [Internet]. 2010 Aug. 12 [cited 2025 Aug. 21];9(1). Available from: https://ojs.ict.unesp.br/index.php/cob/article/view/276

Issue

Vol. 9 No. 1 (2006)

Section

Clinical or Laboratorial Research

License

Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.

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