Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study: in vivo study

Agustin Wulan Suci-Dharmayanti; Zahara Meilawaty; Tantin Ermawati; Agus Murdojohadi  Putradjaka; Zahreni Hamzah

doi:10.4322/bds.2024.e3880

Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study

in vivo study

Authors

Agustin Wulan Suci-Dharmayanti Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. https://orcid.org/0000-0003-3037-7055
Zahara Meilawaty Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. https://orcid.org/0000-0001-7709-4576
Tantin Ermawati Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. https://orcid.org/0000-0002-0322-8997
Agus Murdojohadi Putradjaka Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. https://orcid.org/0009-0006-2261-8647
Zahreni Hamzah Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. https://orcid.org/0000-0001-5101-0872

DOI:

https://doi.org/10.4322/bds.2024.e3880

Abstract

Periodontal diseases and metabolic syndrome are related to complicated multifactorial conditions. However, the relationship is not yet evident. Estrogen insufficiency might correlate to this condition, possibly caused by ovarian removal and Porphyromonas gingivalis (P. gingivalis) infection. This study aimed to evaluate the effect of ovarian dysfunction caused by ovariectomy and P. gingivalis infection to metabolic syndrome development. This study was an experimental laboratory study using female rats Sprague Dawley Strain. Animal models were divided into four groups: control, ovariectomy (OVX), ovariectomy-periodontitis (OPG), and periodontitis (PG). The purpose of every treatment in each group was to induce ovarian dysfunction. The OVX group was undertaken ovaries removal surgery. PG was performed P. gingivalis induction. Therefore OPG was a combination of ovariectomy and P. gingivalis induction. Blood was drawn and observed on days 0, 3, 7, 14, 21, and 28. The blood sample was examined for uric acid, cholesterol, glucose and estrogen. The collected data were all statistically examined. All treatment groups presented body weight and blood biochemical observation significantly higher than the control group, except total cholesterol (p<0.05). Moreover, most variables presented a correlation between groups to body weight and biochemical blood indicators, except blood uric acid level (R>0.5). The metabolic syndrome was triggered by ovarian dysfunction brought on by P. gingivalis infection after ovariectomy. They both took the same risk. Even P. gingivalis induction made metabolic syndrome in the group of animal models which underwent ovariectomy worse.

KEYWORDS

Estrogen deficiency; Metabolic syndrome; Ovariectomy; Ovarian dysfunction; P. gingivalis.

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Published

2024-06-21

Issue

Vol. 27 No. 1 (2024): Jan - Mar / 2024 - Published Jan 2024

Section

Clinical or Laboratorial Research

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All manuscript's copyright ownership of the article "____________________________________________________________________" is transferred from the author(s) to the BRAZILIAN DENTAL SCIENCE, in the event the work is published. The manuscript has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.

We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.

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