Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment

Authors

  • Marcos Sergio Endo Department of Restorative Dentistry - Endodontic Division, Piracicaba Dental School - State University of Campinas - UNICAMP - Piracicaba - SP - Brazil.
  • Fernanda Graziela Corrêa Signoretti Department of Restorative Dentistry - Endodontic Division, Piracicaba Dental School - State University of Campinas - UNICAMP - Piracicaba - SP - Brazil.
  • Vivian Sayuri Kitayama Federal University of Santa Catarina - SC - Brazil.
  • Ariane Cássia Salustiano Marinho Department of Restorative Dentistry - Endodontic Division, Piracicaba Dental School - State University of Campinas - UNICAMP - Piracicaba - SP - Brazil.
  • Frederico Canato Martinho Department of Restorative Dentistry – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Brenda Paula Figueiredo de Almeida Gomes Department of Restorative Dentistry - Endodontic Division, Piracicaba Dental School - State University of Campinas - UNICAMP - Piracicaba - SP - Brazil.

DOI:

https://doi.org/10.14295/bds.2014.v17i3.1016

Abstract

Objective: The aim of this study was to evaluate the minimal inhibitory concentrations (MIC) of different antibiotic agents against to the most prevalent microorganism found in root-filled canals by culture and molecular approaches. Material and Methods: The microbial samples were taken either from thirty root-filled canals after removal of gutta-percha. Culture methods and 16s rDNA assay were used to identify the E faecails present in the samples. The antimicrobial susceptibilities of the isolates of E faecalis were determined by MIC values using the E test System and interpreted according to the Clinical and Laboratory Standards Institute guidelines. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin, rifampicin and moxifloxacin. Results: E faecalis were isolated (7/30) and detected (13/30) by culture and PCR assay, respectively. All tested E faecalis (n=12) were highly sensitive to amoxicillin, moxifloxacin, vancomycin, benzylpenicillin and amoxicillin-clavulanic acid. Some antibiotics were resistant against E faecalis strains such as rifampicin (4/12), tetracycline (2/12), doxycycline (1/12), erythromycin (3/12) and azythromycin (8/12). Conclusion: Amoxicillin, amoxicillin-clavulanic acid, benzylpenicillin, vancomycin and moxifloxacin were the most active antibiotics, in vitro, against E faecalis clinical strains, with all the isolates being susceptible. Azithromycin and erythromycin were least effective, with none percentage of isolates being susceptible, during laboratory testing. Moreover, E faecalis were identified more frequently by PCR assay than by culture technique.

Keywords: Retreatment; Antibiotics, antimicrobial susceptibility; Enterococcus faecalis; Antibiotic resistance. 

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Published

2014-09-15

Issue

Vol. 17 No. 3 (2014): Jul. - Sep. / 2014 - Published August 2014

Section

Clinical or Laboratorial Research

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