Storage protocol of dental pulp cells from human exfoliated deciduous teeth.

Authors

  • Natalino Lourenço Neto Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Letícia Florindo Pereira Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Nádia Carolina Teixeira Marques Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Mariel Prado Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Luciana Lourenço Ribeiro Vitor Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil. http://orcid.org/0000-0003-1671-0851
  • Cintia Kazuko Tokuhara Department of Biological Sciences – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Rodrigo Cardoso Oliveira Department of Biological Sciences – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.
  • Maria Aparecida Andrade Moreira Machado Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil. http://orcid.org/0000-0003-3778-7444
  • Thais Marchini Oliveira Department of Pediatric Dentistry – Orthodontics and Public Health – Bauru School of Dentistry – University of São Paulo – Bauru – São Paulo – Brazil.

DOI:

https://doi.org/10.14295/bds.2017.v20i3.1457

Abstract

Objective: This study aimed to isolate the cells from the dental pulp tissue of human primary teeth, study the capacity of proliferation, characterize the cells and standardize the technique of culture and expansion to create a cell banking. Material and Methods: Primary teeth with no caries and orthodontic reasons were extracted for pulp tissue obtainment. The cells were extracted from the pulp cells, isolated and cultured under ideal conditions until full expansion. Results: After consecutive passages, the cultured cells were characterized using immunofluorescence technique and frozen between the 2nd and 6th passage, thus creating a biorepository of dental pulp cells from human primary teeth. Conclusion: The creation of a cell banking from dental pulp cells from human primary teeth enables the easy application of cells in laboratorial studies, reducing the cost and time for obtaining the samples, avoid the involvement of new subjects and allow a fast reproducibility of the researches.

 

Keywords

Cell culture techniques; Tooth, deciduous; Pulp; Fibroblasts; Cryopreservation.

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Published

2017-09-26

Issue

Vol. 20 No. 3 (2017): Jul. - Sep. / 2017 - Published September 2017

Section

Clinical or Laboratorial Research