Initial and pulp chamber concentration of hydrogen peroxide using different bleaching products

Authors

DOI:

https://doi.org/10.14295/bds.2020.v23i2.1942

Abstract

Objective: This study’s aim was to quantify the hydrogen peroxide (HP) penetration into the pulp chamber of teeth submitted to different protocols of bleaching. Material and Methods: Ninety premolars were randomly divided into nine groups according to the bleaching agent protocol (n = 10): control (no bleaching), carbamide peroxide 10% [10% CP], carbamide peroxide 16% [16% CP], carbamide peroxide 22% [22% CP], hydrogen peroxide 4% [4% HP], hydrogen peroxide 6% [6% HP], hydrogen peroxide 7.5% [7.5% HP], hydrogen peroxide 10% [10% HP] and hydrogen peroxide 35% [35% HP]. The penetration of HP was measured via spectrophotometric analysis of the acetate buffer solution from the pulp chamber. The absorbance of the resulting solution was determined in a spectrophotometer and converted into equivalent concentration of HP (?g/ mL). To analyze the concentration of HP, the titration of bleaching agents with potassium permanganate was used. Data were subjected to ANOVA and Tukey’s test for pairwise comparison (? = 0.05). Results: Higher concentration of HP in the pulp chamber was found in the HP 35% group (p < 0.0001). No significant difference between at-home protocols were observed (p = 0.64). Titration values showed that the concentration of the products was similar to that claimed by the manufacturer. Conclusion: It follows that the amount of HP that reaches the pulp chamber is not proportional to the concentration of whitening gels, but depends on the application time recommended by the manufacturers.

KEYWORDS

At-home bleaching; Dental enamel permeability; Inoffice bleaching; Tooth bleaching.

 

References

Theobald AH, Wong BK, Quick AN, Thomson WM. The impact of the popular media on cosmetic dentistry. N Z Dent J. 2006; 102 (3): 58-63.

Joiner A. Review of the effects of peroxide on enamel and dentine properties. J Dent. 2007; 35 (12): 889-896.

Mokhlis GR, Matis BA, Cochran MA, Eckert GJ. A clinical evaluation of carbamide peroxide and hydrogen peroxide whitening agents during daytime use. J Am Dent Assoc. 2000; 131 (9): 1269-1277.

Haywood VB.Treating sensitivity during tooth whitening. Compend Contin Educ Dent. 2005; 26 (9 Suppl 3): 11-20.

American Dental Association: Tooth whitening/bleaching: treatment considerations for dentists and their patients ADA Council on Scientific Affairs Chicago; 2009.

Boushell LW, Ritter AV, Garland GE, et al. Nightguard vital bleaching: side effects and patient satisfaction 10 to 17 years post-treatment. i. 2012; 24 (3): 211-219. doi: 10.1111/j.1708-8240.2011.00479.x

Tay LY, Kose C, Herrera DR, Reis A, Loguercio AD. Long-term efficacy of in-office and at-home bleaching: a 2-year double-blind randomized clinical trial. Am J Dent. 2012; 25 (4): 199-204.

Rezende M, Loguercio AD, Kossatz S , Reis A. Predictive factors on the efficacy and risk/intensity of tooth sensitivity of dental bleaching: A multi regression and logistic analysis. J Dent. 2016; 45: 1-6. doi: 10.1016/j.jdent.2015.11.003.

Cardenas AFM, Maran BM, Araújo LCR, de Siqueira FSF, Wambier LM, Gonzaga CC, Loguercio AD, Reis A. Are combined bleaching techniques better than their sole application? A systematic review and meta-analysis. Clin Oral Investig. 2019;23(10):3673-3689. doi: 10.1007/s00784-019-03042-4.

Basting RT, Amaral FL, Franca FM, Florio FM. Clinical comparative study of the effectiveness of and tooth sensitivity to 10% and 20% carbamide peroxide home-use and 35% and 38% hydrogen peroxide in-office bleaching materials containing desensitizing agents. Oper Dent. 2012; 37 (5): 464-473. doi:10.2341/11-337-C.

Briso AL, Goncalves RS, Costa FB, Gallinari MO, Cintra LT, Santos PH. Demineralization and hydrogen peroxide penetration in teeth with incipient lesions. Braz Dent J. 2015; 26 (2): 135-40. doi: 10.1590/0103-6440201300225.

Parreiras S, Mena-Serrano A, Moreira CG, Otuki M, Loguercio D, Reis A. Penetration and cytotoxicity of a bleaching gel activated by LED/laser in restored teeth. Am J Dent. 2014; 27 (6): 301-306.

Soares DG, Basso FG, Pontes EC, Garcia Lda F, Hebling J, de Souza Costa CA. Effective tooth-bleaching protocols capable of reducing H(2)O(2) diffusion through enamel and dentine. J Dent. 2014; 42 (3): 351-358. doi: 10.1016/j.jdent.2013.09.001.

Cooper JS, Bokmeyer TJ, Bowles WH. Penetration of the pulp chamber by carbamide peroxide bleaching agents. J Endod. 1992; 18 (7): 315-317.

Bowles WH, Ugwuneri Z. Pulp chamber penetration by hydrogen peroxide following vital bleaching procedures. J Endod. 1987; 13 (8): 375-377.

Mendham J, Afonso JC. Vogel Quantitative chemical analysis. 1st ed. Livros Tecnicos e Científicos Editora S.A., 2002.

Mena-Serrano AP, Parreiras SO, do Nascimento EM, et al. Effects of the concentration and composition of in-office bleaching gels on hydrogen peroxide penetration into the pulp chamber. Oper Dent. 2015; 40 (2): 76-82. doi: 10.2341/13-352-L.

Marson FC, Goncalves RS, Silva CO, et al. Penetration of hydrogen peroxide and degradation rate of different bleaching products. Oper Dent. 2015; 40 (1): 72-79. doi: 10.2341/13-270-L.

Gokay O, Mujdeci A, Algin E. In vitro peroxide penetration into the pulp chamber from newer bleaching products. Int Endod J. 2005; 38 (8): 516-520.

Hegedus C, Bistey T, Flora-Nagy E, Keszthelyi G, Jenei A. An atomic force microscopy study on the effect of bleaching agents on enamel surface. J Dent. 1999; 27 (7): 509-515.

Acunã ED, Parreiras SO, Favoreto MW, et al. In-Office bleaching with a commercial 40% hydrogen peroxide fel modified to have different pHs: Color change, surface morphology, and penetration of hydrogen peroxide into theu pulp chamber . J Esthet Restor Dent. 2019; 31 (1): 1-6. doi:10.1007/s00784-014-1285-3.

de Geus JL, Wambier LM, Boing TF, Loguercio AD, Reis A. At-home bleaching with 10% vs more concentrated carbamide peroxide gels: A systematic review and meta-analysis. Oper Dent. 2018; 43 (4): 210-222.

Meireles SS, Heckmann SS, Leida FL, dos Santos Ida S, Della Bona A, Demarco FF. Efficacy and safety of 10% and 16% carbamide peroxide tooth-whitening gels: a randomized clinical trial. Oper Dent. 2008; 33 (6): 606-612.

Braun A, Jepsen S, Krause F. Spectrophotometric and visual evaluation of vital tooth bleaching employing different carbamide peroxide concentrations. Dent Mater. 2007; 23 (2): 165-169.

Krause F, Jepsen S, Braun A. Subjective intensities of pain and contentment with treatment outcomes during tray bleaching of vital teeth employing different carbamide peroxide concentrations. Quintessence Int. 2008; 39 (3):203-209. doi:10.2341/07-150.

Delgado E, Hernandez-Cott PL, Stewart B, Collins M, De Vizio W. Tooth-whitening efficacy of custom tray-delivered 9% hydrogen peroxide and 20% carbamide peroxide during daytime use: a 14-day clinical trial. P R Health Sci J. 2007; 26 (4): 367-372.

Ziebolz D, Helms K, Hannig C, Attin T. Efficacy and oral side effects of two highly concentrated tray-based bleaching systems. Clin Oral Investig. 2007; 11 (3): 267-275.

Alonso de la Pena V, Lopez Raton M. Randomized clinical trial on the efficacy and safety of four professional at-home tooth whitening gels. Oper Dent. 2014; 39 (2): 136-143.

Featherstone JD. The continuum of dental caries—evidence for a dynamic disease process. J Dent Res. 2004; 83 Spec No C:C 39-42.

Kensche A, Pötschke S, Hannig C, Richter G, Hoth-Hannig W, Hannig M. Influence of Calcium Phosphate and Apatite Containing Products on Enamel Erosion. ScientificWorldJournal. 2016; ID7959273. doi: 10.2341/12-402-C.

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Published

2020-03-31

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Clinical or Laboratorial Research Manuscript