Effect of erosive challenge and cigarette smoke on dentin microhardness, surface morphology and bond strength





Objective: This study aimed to evaluate the surface microhardness and morphology, as well as the microshear bond strength of a self-etching adhesive (Clearfil SE, Kuraray) to eroded dentin, exposed or not to cigarette smoke. Forty dental crowns were divided into 4 groups (n = 10): no treatment (control) (C); erosion (E); erosion + cigarette smoke exposure (ES); cigarette smoke exposure (S). Samples were prepared through third molars polishing until dentin exposure, followed by crown section. Erosive cycles were performed 5 times/day for 30 s at 60 min intervals. Cigarette smoke was produced with twenty cigarettes/day, during 5 days. Microhardness was evaluated initially and after the treatments. Microshear bond strength was tested after the treatments and dentin restoration with flow composite. Failure patterns and dentin morphology was evaluated by Scanning Electron Microscopy. Microshear bond strength data was submitted to two-way ANOVA, microharness test was adjusted by gamma distribution to be a non-parametric analyses (p=0.05), and surface morphology as qualitative analyses. Loss percentage of microhardness was observed only in groups submitted to erosion. Bond strength was statistically similar between all groups. The most prevalent failure pattern was of adhesive type. Morphological analysis of dentin showed obliterated tubules in groups submitted to cigarette smoke exposure. Cigarette smoke exposure did not promote any effect in the percentage of microhardness loss, as in sound dentin as in eroded dentin. Cigarette smoke, erosion, and association of both, did not alter the bond strength of self-etching adhesives to dentin.


Bond strength; Cigarette Smoke; Dental erosion; Microhardness; Self-etching adhesives.

Author Biographies

Marcela Alvarez Ferretti, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD student, Department of Restorative Dentistry

Jéssica Dias Theobaldo, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD, Department of Restorative Dentistry

Renata Pereira, Piracicaba Dental School- University of Campinas

DDS, MSc, PhD student, Department of Restorative Dentistry

Waldemir Francisco Vieira-Junior, São Leopoldo Mandic Institute and Dental Research Center Campinas - Campinas - SP - Brazil.

DDS, MSc, PhD, Professor, Restorative Dentistry

Glaucia Maria Bovi Ambrosano, Department of Bioscience - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

Agronomist, MSc, PhD,Full Professor, Department of Bioscience

Flávio Henrique Baggio Aguiar, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD, Associate Professor, Department of Restorative Dentistry,


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Clinical or Laboratorial Research Manuscript