Effect of erosive challenge and cigarette smoke on dentin microhardness, surface morphology and bond strength





Objective: This study aimed to evaluate the surface microhardness and morphology, as well as the microshear bond strength of a self-etching adhesive (Clearfil SE, Kuraray) to eroded dentin, exposed or not to cigarette smoke. Forty dental crowns were divided into 4 groups (n = 10): no treatment (control) (C); erosion (E); erosion + cigarette smoke exposure (ES); cigarette smoke exposure (S). Samples were prepared through third molars polishing until dentin exposure, followed by crown section. Erosive cycles were performed 5 times/day for 30 s at 60 min intervals. Cigarette smoke was produced with twenty cigarettes/day, during 5 days. Microhardness was evaluated initially and after the treatments. Microshear bond strength was tested after the treatments and dentin restoration with flow composite. Failure patterns and dentin morphology was evaluated by Scanning Electron Microscopy. Microshear bond strength data was submitted to two-way ANOVA, microharness test was adjusted by gamma distribution to be a non-parametric analyses (p=0.05), and surface morphology as qualitative analyses. Loss percentage of microhardness was observed only in groups submitted to erosion. Bond strength was statistically similar between all groups. The most prevalent failure pattern was of adhesive type. Morphological analysis of dentin showed obliterated tubules in groups submitted to cigarette smoke exposure. Cigarette smoke exposure did not promote any effect in the percentage of microhardness loss, as in sound dentin as in eroded dentin. Cigarette smoke, erosion, and association of both, did not alter the bond strength of self-etching adhesives to dentin.


Bond strength; Cigarette Smoke; Dental erosion; Microhardness; Self-etching adhesives.

Author Biographies

Marcela Alvarez Ferretti, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD student, Department of Restorative Dentistry

Jéssica Dias Theobaldo, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD, Department of Restorative Dentistry

Renata Pereira, Piracicaba Dental School- University of Campinas

DDS, MSc, PhD student, Department of Restorative Dentistry

Waldemir Francisco Vieira-Junior, São Leopoldo Mandic Institute and Dental Research Center Campinas - Campinas - SP - Brazil.

DDS, MSc, PhD, Professor, Restorative Dentistry

Glaucia Maria Bovi Ambrosano, Department of Bioscience - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

Agronomist, MSc, PhD,Full Professor, Department of Bioscience

Flávio Henrique Baggio Aguiar, Department of Restorative Dentistry - Piracicaba Dental School - University of Campinas - Piracicaba - SP - Brazil.

DDS, MSc, PhD, Associate Professor, Department of Restorative Dentistry,


. OMS 2015 World Health Organization. Who Report on The Global Tabacco Epidemic, Raising taxes on tobacco. 2015

. World Health Organization. WHO global report on trends in prevalence of tobacco smoking 2000-2025, second edition. Geneva. 2018. ISBN 978-92-4-151417-0

. Vellappally S, Fiala Z, Smejkalová J, Jacob V, Somanathan R. Smoking related systemic and oral diseases. Acta Medica (Hradec Kralove) 2007; 50(3):161-6.

. Pinzon LM, Oguri M, O'Keefe K, Dusevish V, Spencer P, et al. Bond strength of adhesives to dentin contaminated with smoker's saliva. Odontology. 2010; 98(1):37-43.

. Takeuchi CY, Corrêa-Afonso AM, Pedrazzi H, Dinelli W, Palma-Dibb RG. Deposition of lead and cadmium released by cigarette smoke in dental structures and resin composite. Microsc Res Tech. 2011; 74(3):287-91.

. Mathias P, Costa L, Saraiva LO, Rossi TA, Cavalcanti AN, et al. Morphologic texture characterization allied to cigarette smoke increase pigmentation in composite resin restorations. J Esthet Restor Dent. 2010; 22(4):252-9.

. Theobaldo JD, Catelan A, Rodrigues-Filho U, Marchi GM, Lima D et al. Effect of Cigarette Smoke on Resin Composite Bond Strength to Enamel and Dentin Using Different Adhesive Systems. Oper Dent. 2016; 41(3): E57-63.

. Almeida e Silva JS, de Araujo EM Jr, Araujo E. Cigarette smoke affects bonding to dentin. General Dentistry. 2010; 58(4): 326-330

. Chu CH, Lam A, Lo EC. Dentin Hypersensitivity and its manegament. Gen Dent. 2010; 59: 115-122.

. Al-Dlaigan YH, Shaw L,Smith A. Dental erosion in a group of British 14-year-old school children Part II: Influence of dietary intake. British Dental Journal. 2001; 190: 259-261

. Clapp O, Morgan MZ, Fairchild RM. Br Dent J.The top five selling UK energy drinks: implications for dental and general health. 2019 ;226(7):493-97. doi: 10.1038/s41415-019-0114-0;

. Nguyen Ngoc C, Ghuman T, Ahmed SN, Donovan TE. The erosive potential of additive artificial flavoring in bottled water.. Gen Dent. 2018; 66(5):46-51

. Ten Cate JM, Imfeld T. Dental erosion, summary. Eur J Oral Sci.1996; 104(2):241-4.

. O'Toole S, Bartlett D. The relationship between dentine hypersensitivity, dietary acid intake and erosive tooth wear. J Dent. 2017; 67:84-7. doi: 10.1016/j.jdent.2017.10.002

. Meng D, Sang-Wan S, Min-Soo K, Jae-Jun R, Jeong-Yol L. The effect of a desensitizer and CO2 laser irradiation on bond performance between eroded dentin and resin composite. J Adv Prosthodont. 2014; 6: 165-170

. Bartlett DW, Evans DF, Smith BG. Oral regurgitation after reflux provoking meals: a possible cause of dental erosion? Journal of Oral Rehabilitation. 1997; 24: 102-108.

. Manuja N, Nagpal R, Pandit IK. Dental Adhesion: Mechanism, Techniques and Durability. Journal Clinical Pediatric Dentistry. 2012; 36(3) 223-234.

. Xie J, Powers JM, McGuckin RS. In vitro bond strength of two adhesives to enamel and dentin under normal and contaminated conditions. Dent Mater. 1993; 9:295-299

. Theobaldo JD, Vieira-Junior WF, Catelan A, Mainardi MDCA, Ysnaga OA et al. Effect of Heavy Metals Contamination from Cigarette Smoke on Sound and Caries-Like Enamel. Microsc Microanal. 2018; 24(6):762-767.

. Guedes AP, Moda MD, Suzuki TY, Godas AG, Sundfeld RH, Briso AL, Santos PH. Effect of Fluoride-Releasing Adhesive Systems on the Mechanical Properties of Eroded Dentin. Braz Dent J. 2016; 27(2):153-9. doi: 10.1590/0103-644020160635

. Bertoldo CES, Miranda DA, Souza Junior EJ, Aguiar FHB; Lima DANL; Ferreira RL, Claes I, Lovadino JR. Surface hardness and color change of dental enamel exposed to cigarette smoke. International Journal of Dental Clinics. 2011; 3: 1-4

. Shellis RP, Featherstone JD, Lussi A. Understanding the chemistry of dental erosion. Monogr Oral Sci. 2014;25:163-79. doi: 10.1159/000359943. Epub 2014 Jun 26. Review. PubMed PMID: 24993265.

. Lussi A, Jaeggi T, Zero D. The role of diet in the aetiology of dental erosion. Caries Res. 2004;38 Suppl 1:34-44. Review

. Featherstone JDB. The science and practice of caries prevention. J Am Dent Assoc. 2000; 131: 887–899

. Zimmerli B, De Munck J, Lussi A, Lambrechts P, Van Meerbeek B. Long-term bonding to eroded dentin requires superficial bur preparation. Clin Oral Investig. 2012; 16(5):1451-61.

. Cruz JB, Lenzi TL, Tedesco TK, Guglielmi Cde A, Raggio DP. Eroded dentin does not jeopardize the bond strength of adhesive restorative materials. Braz Oral Res. 2012; 26(4):306-12.

. Giannini M, Makishi P, Ayres AP, Vermelho PM, Fronza BM, Nikaido T, Tagami J. Self-etch adhesive systems: a literature review. Braz Dent J. 2015;26(1):3-10. doi: 10.1590/0103-6440201302442. Review

. Yoshida Y, Van Meerbeek B, Nakayama Y, Yoshioka M, Snauwaert J, Abe Y, et al. Adhesion to and decalcification of hydroxyapatite by carboxylic acids. Journal of dental research. 2001; 80(6): 1565-1569.

. Fukegawa D, Hayakawa S, Yoshida Y, Suzuki K, Osaka A, Van Meerbeek B. Chemical interaction of phosphoric acid ester with hydroxyapatite. Journal of dental research. 2006; 85(10): 941-4.

. Matsui N, Takagaki T, Sadr A, Ikeda M, Ichinose S, Nikaido T, et al. The role of MDP in a bonding resin of a two-step self-etching adhesive system. Dental materials. 2015; 34(2): 227-33.

. Yoshida Y, Nagakane K, Fakuda R, Nakayama Y, Okazaki M, Shintani H et al. Comparative Study on Adhesive Performance of Functional Monomers. J Dent Res. 2004); 83: 454-458

. Turp V, Sen D, Tuncelli B, Ozcan M. Adhesion of 10-MDP containing resin cements to dentin with and without the etch-and-rinse technique. The journal of advanced prosthodontics. 2013; 5(3): 226-33

. Cruz JB, Bonini G, Lenzi TL, Imparato JCP, Raggio DP. Bonding stability of adhesive systems to eroded dentin. Braz Oral Res. 2015; 29. pii: S1806-83242015000100284.

. Flury S, Koch T, Peutzfeldt A, Lussi A, Ganss C.The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin. Clin Oral Investig. 2013; 17(1):217-25. doi: 10.1007/s00784-012-0697-1.

. Flury S, Lussi A, Peutzfeldt A. Long-Term Bond Strength of Two Benzalkonium Chloride-Modified Adhesive Systems to Eroded Dentin. Biomed Res Int. 2017;2017:1207208. doi: 10.1155/2017/1207208.

. Wang Y & Spencer P. Effect of acid etching time and technique on interfacial characteristics of the adhesive-dentin bond using differential staining. Eur J Oral Sci. 2004; 112(3):293-9.

. Sattabanasuk V, Shimada Y, Tagami J. Bonding of resin to artificially carious dentin. J Adhes Dent Autumn. 2005; 7(3):183-92.






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