Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats
DOI:
https://doi.org/10.14295/bds.2021.v24i2.2394Abstract
Objective: To evaluate the effect of salbutamol, montelukast, and prednisone on orthodontic tooth movement in rats. Material and Methods: In vivo experimental preclinical study. The sample consisted of 48 rats randomly distributed in four study groups. Group A was given saline solution; to group B, salbutamol 4 mg/Kg; to group C, montelukast 2.5 mg/Kg and to group D, prednisone 2.5 mg/Kg. All were fitted with orthodontic devices and the medications were administered intraperitoneally every 12 hours for 5 days. The clinical evaluation (variation in the interincisal distance) was performed at one, three, five, and seven days and the histopathological analysis (cell count) at five and seven days. Results: In the clinical evaluation of the variation in the interincisal distance, a significant difference was found in all the evaluations (p <0.05). It was found that the salbutamol group presented higher variation values in the interincisal distance on all the days evaluated. In the histopathological analysis at five and seven days, it was found that the osteoblast and osteocyte count was significantly higher in the salbutamol group compared to the other groups (p ><0.05). However, in the subgroup analysis, it was found that there was no significant difference in the osteoblast and osteocyte count between the prednisone, montelukast, and control group (p>0.05). Conclusion: The administration of salbutamol increased the magnitude of orthodontic tooth movement; nonetheless, the administration of montelukast and prednisone did not modify the magnitude of orthodontic tooth movement in rats.
Keywords
Albuterol; Montelukast; Prednisone; Rats; Tooth movement.
References
Alqahtani H. Medically compromised patients in orthodontic practice: Review of evidence and recommendations. Int Orthod. 2019 Dec;17(4):776–88. https://doi.org/10.1016/j.ortho.2019.08.015
Inagaki Y, Akahane M, Shimizu T, Inoue K, Egawa T, Kira T, et al. Modifying oxygen tension affects bone marrow stromal cell osteogenesis for regenerative medicine. World J Stem Cells. 2017;9(7):98–106. https://dx.doi.org/10.4252/wjsc.v9.i7.98
Chumpitaz-Cerrate V, Bellido-Meza JA, Chavez-Rimache L, Rodriguez-Vargas C. Impact of inhaler use on dental caries in asthma pediatrics patients: A case-control study. Arch Argent Pediatr. 2020 Feb;118(1):38–46. http://dx.doi.org/10.5546/aap.2020.eng.38
Bartzela T, Turp JC, Motschall E, Maltha JC. Medication effects on the rate of orthodontic tooth movement: a systematic literature review. Am J Orthod Dentofacial Orthop. 2009 Jan;135(1):16–26. https://doi.org/10.1016/j.ajodo.2008.08.016
Asaad H, Al-Sabbagh R, Al-Tabba D, Kujan O. Effect of the leukotriene receptor antagonist montelukast on orthodontic tooth movement. J Oral Sci. 2017;59(2):297–302. https://doi.org/10.2334/josnusd.16-0482
Sato AY, Tu X, McAndrews KA, Plotkin LI, Bellido T. Prevention of glucocorticoid induced-apoptosis of osteoblasts and osteocytes by protecting against endoplasmic reticulum (ER) stress in vitro and in vivo in female mice. Bone. 2015 Apr;73:60–8. https://doi.org/10.1016/j.bone.2014.12.012
Machado CC, Nojima Mda C, Rodrigues e Silva PM, Mandarim-de-Lacerda CA. Histomorphometric study of the periodontal ligament in the initial period of orthodontic movement in Wistar rats with induced allergic asthma. Am J Orthod Dentofacial Orthop. 2012 Sep;142(3):333–8.
https://doi.org/10.1016/j.ajodo.2012.04.011
Chibebe PC, Starobinas N, Pallos D. Juveniles versus adults: differences in PGE2 levels in the gingival crevicular fluid during orthodontic tooth movement. Braz Oral Res. 2010 Mar;24(1):108–13. http://dx.doi.org/10.1590/S1806-83242010000100018
Liang H, Zeng Y, Feng Y, Wu H, Gong P, Yao Q. Selective beta2-adrenoreceptor signaling regulates osteoclastogenesis via modulating RANKL production and neuropeptides expression in osteocytic MLO-Y4 cells. J Cell Biochem. 2018;1-10. https://doi.org/10.1002/jcb.27998
Yao Q, Liang H, Huang B, Xiang L, Wang T, Xiong Y, et al. Beta-adrenergic signaling affect osteoclastogenesis via osteocytic MLO-Y4 cells’ RANKL production. Biochem Bioph Res Co. 2017;488(4):634–40. https://doi.org/10.1016/j.bbrc.2016.11.011
Cottrell J, O'Connor JP. Effect of Non-Steroidal Anti-Inflammatory Drugs on Bone Healing. Pharmaceuticals (Basel, Switzerland). 2010;3(5):1668-93. https://doi.org/10.3390/ph3051668
Nie Z, Chen S, Peng H. Glucocorticoid induces osteonecrosis of the femoral head in rats through GSK3β-mediated osteoblast apoptosis. Biochem Bioph Res Co. 2019 Apr 9;511(3):693-699. https://doi.org/10.1016/j.bbrc.2019.02.118
Garcia-Martinez O, Diaz-Rodriguez L, Rodriguez-Perez L, De Luna-Bertos E, Reyes Botella C, Ruiz CC. Effect of acetaminophen, ibuprofen and methylprednisolone on different parameters of human osteoblast-like cells. Arch Oral Biol. 2011 Apr;56(4):317–23. https://doi.org/10.1016/j.archoralbio.2010.10.018
Krishnan V, Vijayaraghavan N, Manoharan M, Raj J, Davidovitch Z. The Effects of Drug Intake by Patients on Orthodontic Tooth Movement. Seminars in Orthodontics. 2012;18(4):278–85. https://doi.org/10.1053/j.sodo.2012.06.006
ARRIVE guidelines | NC3Rs [Internet]. [cited 2020 May 8]. Available from: https://www.nc3rs.org.uk/arrive-guidelines.
National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals [Internet]. 8th ed. Washington (DC): National Academies Press (US); 2011 [cited 2020 May 8]. (The National Academies Collection: Reports funded by National Institutes of Health). Available from: http://www.ncbi.nlm.nih.gov/books/NBK54050/
Novaes A, Desiderá A, Nascimento G and Leite-Panissi C. Effects of Sodium Diclofenac on the Distribution of Fos Protein in Central Amygdala and Lateral Hypothalamus during Experimental Tooth Movement in Rats. World J Neurosci. 2014;4(1):183-189. doi: 10.4236/wjns.2014.42021.
Gonçalves CF, Desiderá AC, do Nascimento GC, Issa JP, Leite-Panissi CR. Experimental tooth movement and photobiomodulation on bone remodeling in rats. Lasers Med Sci. 2016;31(9):1883-1890. doi:10.1007/s10103-016-2064-y
Magdalena CM., Navarro VP, Park DM, Stuani MB and Rocha MJ. c-Fos Expression in Rat Brain Nuclei Following Incisor Tooth Movement. J Dent Res. 2004;83(1):50-54. http://dx.doi.org/10.1177/154405910408300110
Kondo M, Kondo H, Miyazawa K, Goto S, Togari A. Experimental tooth movement-induced osteoclast activation is regulated by sympathetic signaling. Bone. 2013 Jan;52(1):39–47. https://doi.org/10.1016/j.bone.2012.09.007
Uchibori S, Sekiya T, Sato T, Hayashi K, Takeguchi A, Muramatsu R, et al. Suppression of tooth movement-induced sclerostin expression using β-adrenergic receptor blockers. Oral Diseases. 2020;26(3):621–9. https://doi.org/10.1111/odi.13280
De Oliveira EL, Freitas FF, de Macedo CG, Clemente-Napimoga JT, Silva MBF, Manhães-Jr LRC, et al. Low dose propranolol decreases orthodontic movement. Arch Oral Biol. 2014;59(10):1094–100. https://doi.org/10.1016/j.archoralbio.2014.06.006
Kang JH, Ting Z, Moon MR, Sim JS, Lee JM, Doh KE, et al. 5-Lipoxygenase inhibitors suppress RANKL-induced osteoclast formation via NFATc1 expression. Bioorg Med Chem. 2015 Nov 1;23(21):7069–78. https://doi.org/10.1016/j.bmc.2015.09.025
Moura AP, Taddei SR, Queiroz-Junior CM, Madeira MF, Rodrigues LF, Garlet GP, et al. The relevance of leukotrienes for bone resorption induced by mechanical loading. Bone. 2014 Dec;69:133–8. https://doi.org/10.1016/j.bone.2014.09.019
Bergström I, Isaksson H, Koskela A, Tuukkanen J, Ohlsson C, Andersson G, et al. Prednisolone treatment reduces the osteogenic effects of loading in mice. Bone. 2018;112:10–8. https://doi.org/10.1016/j.bone.2018.04.002
Yang J, Li J, Cui X, Li W, Xue Y, Shang P, et al. Blocking glucocorticoid signaling in osteoblasts and osteocytes prevents mechanical unloading-induced cortical bone loss. Bone. 2020 Jan;130:115108. https://doi.org/10.1016/j.bone.2019.115108
Piemontese M, Onal M, Xiong J, Wang Y, Almeida M, Thostenson JD, et al. Suppression of autophagy in osteocytes does not modify the adverse effects of glucocorticoids on cortical bone. Bone. 2015;75:18–26. https://doi.org/10.1016/j.bone.2015.02.005
Wood CL, Soucek O, Wong SC, Zaman F, Farquharson C, Savendahl L, et al. Animal models to explore the effects of glucocorticoids on skeletal growth and structure. J Endocrinol. 2018 Jan;236(1):R69-r91. https://doi.org/10.1530/JOE-17-0361
