Effectiveness freeze dried platelet rich plasma combination of hyaluronic acid after periodontal surgical healing: an experimental study on animal model

(In Vivo Studies)





Objective: the study aimed to investigate the effectiveness of the combined application freeze-dried platelet-rich plasma (FD-PRP) and hyaluronic acid (HA) in healing after periodontal surgery. Material and Methods: This is an experimental laboratory research with a design pre and post-test only control group design. Freeze-dried platelet-rich plasma and HA were applied at periodontal surgical sites in periodontitis induced Wistar rats’ mandibulary incisors. Bleeding on probing (BOP) was observed as a clinical aspect. Porphyromonas gingivalis was examined and counted. Meanwhile, the healing process was seen by examining fibroblasts and Platelet-Derived Growth Factor-BB (PDGF-BB) in histology and immunohistochemistry. In this study, BOP decreased faster after applying FD-PRP and HA. Data were processed by ANOVA and Kruskal-Wallis test. Results: Clinical examination by measuring BOP level showed better inflammation resolution at 14th day (p=0.007). Porphyromonas gingivalis (P. gingivalis) count was also reduced in the treatment group (p=0.001 at 3rd and 14th day). The histology aspect by measuring fibroblast count (p=0.025 at 3rd day and p=0.001 at 14th day) and immunohistochemistry by measuring PDGF-BB expression (p=0.014 at 3rd day and p=0.030 at 14th day) showed better tissue healing. Conclusion: combination of FD-PRP and HA has accelerated the healing of periodontal surgical wounds.


Fibroblast; Hyaluronic acid; Periodontitis; Platelet-rich plasma; Porphyromonas gingivalis.






Clinical or Laboratorial Research Manuscript