Effectiveness freeze dried platelet rich plasma combination of hyaluronic acid after periodontal surgical healing: an experimental study on animal model: (In Vivo Studies)

Pitu Wulandari; Denny  Satria; Martina Amalia; Budi; Rio Simanjuntak

doi:10.4322/bds.2023.e3680

Effectiveness freeze dried platelet rich plasma combination of hyaluronic acid after periodontal surgical healing: an experimental study on animal model

(In Vivo Studies)

Authors

Pitu Wulandari Universitas Sumatera Utara, Faculty of Dentistry, Department of Periodontics, Medan, Indonesia https://orcid.org/0000-0001-8337-2691
Denny Satria Universitas Sumatera Utara, Department of Pharmaceutical Biology, Medan, Indonesia https://orcid.org/0000-0003-4724-3256
Martina Amalia Universitas Sumatera Utara, Faculty of Dentistry, Department of Periodontics, Medan, Indonesia https://orcid.org/0000-0003-3252-2033
Budi Universitas Sumatera Utara, Faculty of Dentistry, Periodontics Residency Program, Medan, Indonesia https://orcid.org/0000-0002-1452-5399
Rio Simanjuntak Universitas Sumatera Utara, Faculty of Dentistry, Periodontics Residency Program, Medan, Indonesia https://orcid.org/0000-0002-7546-2600

DOI:

https://doi.org/10.4322/bds.2023.e3680

Abstract

Objective: the study aimed to investigate the effectiveness of the combined application freeze-dried platelet-rich plasma (FD-PRP) and hyaluronic acid (HA) in healing after periodontal surgery. Material and Methods: This is an experimental laboratory research with a design pre and post-test only control group design. Freeze-dried platelet-rich plasma and HA were applied at periodontal surgical sites in periodontitis induced Wistar rats’ mandibulary incisors. Bleeding on probing (BOP) was observed as a clinical aspect. Porphyromonas gingivalis was examined and counted. Meanwhile, the healing process was seen by examining fibroblasts and Platelet-Derived Growth Factor-BB (PDGF-BB) in histology and immunohistochemistry. In this study, BOP decreased faster after applying FD-PRP and HA. Data were processed by ANOVA and Kruskal-Wallis test. Results: Clinical examination by measuring BOP level showed better inflammation resolution at 14th day (p=0.007). Porphyromonas gingivalis (P. gingivalis) count was also reduced in the treatment group (p=0.001 at 3rd and 14th day). The histology aspect by measuring fibroblast count (p=0.025 at 3rd day and p=0.001 at 14th day) and immunohistochemistry by measuring PDGF-BB expression (p=0.014 at 3rd day and p=0.030 at 14th day) showed better tissue healing. Conclusion: combination of FD-PRP and HA has accelerated the healing of periodontal surgical wounds.

KEYWORDS

Fibroblast; Hyaluronic acid; Periodontitis; Platelet-rich plasma; Porphyromonas gingivalis.

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Published

2023-06-23

Issue

Vol. 26 No. 2 (2023): Apr - Jun / 2023 - Published Mar 2023

Section

Clinical or Laboratorial Research

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We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.

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