Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans

Authors

  • Juliana Guimarães Santos Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Escola de Odontologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, SP, Brazil. https://orcid.org/0000-0002-2531-2102
  • Isabela Amêndola Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Escola de Odontologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, SP, Brazil. https://orcid.org/0000-0002-0010-9757
  • Ellen Roberta Lima Bessa ICT-UNESP
  • Grazialla Nuernberg Back-Brito Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Escola de Odontologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, SP, Brazil. https://orcid.org/0000-0003-3255-4289
  • Luciane Dias Oliveira https://orcid.org/0000-0001-9956-7768

DOI:

https://doi.org/10.4322/bds.2024.e4039

Abstract

Objective: Analyze the phenotypic expression of virulence factors in Candida albicans biofilms against plant glycolic extracts. Material and Methods: The biofilms of Candida albicans (ATCC 18804) obtained from incubation for 48 hours were exposed for 5 minutes and 24 hours to different concentrations of glycolic extracts of Hamamelis virginiana and Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M, in order to verify the antifungal activity of the proteinase, phospholipase and hemolysin. Results: All extracts were effective in reducing biofilm. In contact for 5 minutes. the extracts reduced 50% of the biofilm. After 24 hours, the Persea americana extract showed the biofilm at 90%, followed by Cynara scolymus, which interrupted it at 85%, There was a change in proteinase intensity after 5 minutes and 24 hours. with an average enzymatic activity of 0.69 compared to the control of 0.49. Cynara scolymus was the extract with the highest mean concentration of 100 mg/ml; the phospholipase intensity was changed with Stryphnodendron barbatiman being more effective in 24 hours compared to the control (p< 0.0001). The hemolysin secretion was modified by Hamamelis virginiana (12.5 mg/ml) after 5 minutes of exposure, and in 24 hours. all extracts were capable to cause changes in secretion. Conclusion: The tested extracts have antifungal potential in Candida albicans biofilms, implying a significant reduction in virulence factors. Thus, these can be indicated as an alternative therapeutic tool to reduce the morbidity of these infections, as in both investigated exposure times. they were able to reduce the enzymatic secretion of the fungus.

KEYWORDS

Antifungal agents; Candida albicans; Infection; Plant extracts; Virulence factors.

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Published

2024-03-05

Issue

Vol. 27 No. 1 (2024): Jan - Mar / 2024 - Published Jan 2024

Section

Clinical or Laboratorial Research

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