Antibiofilm activity in vitro of Rosmarinus officinalis and Syzygium cumini glycolic extracts on Staphylococcus spp. of dentistry interest

Authors

  • Fernanda Freire São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Cristiane Aparecida Pereira São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Luciane Dias Oliveira São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Juliana Campos Junqueira São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Antonio Olavo Cardoso Jorge São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2017.v20i2.1430

Abstract

Objective: The aim of this study was to identify the slime production and evaluate the effects of Rosmarinus officinalis (rosemary) and Syzygium cumini (jambolan) glycolic extracts, and 0.12% chlorhexidine (CHX) in biofilms formed by strains of coagulase-positive Staphylococcus - CPS and coagulase negative Staphylococcus - CNS isolated from the oral cavity. Material and Methods: Slime production was evaluated by two methods: the color of colony presented in Congo red agar, and through the amount of slime adhered to polystyrene. Biofilms were grown in acrylic resin discs immersed in broth, inoculated with microbial suspension (106 cells/ml) and incubated at 37°C/48 h. After formation, the biofilms were exposed for 5 minutes to glycol extracts, CHX or saline solution. The viability of biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, and analyzed statistically by Tukey test (p <0.05). Results: The strains S. aureus, S. schleiferi and S. epidermidis obtained the highest values of slime adhered to polystyrene. R. officinalis promoted reductions ranging from 12.1% to 78.7% in biofilms formed by isolates of CPS, and 9.2% to 73.7% in the biofilms of CNS. S. cumini reduced 12% to 55.7% in biofilms of CPS, and 7.9% to 71.5% in biofilms of CNS. With exception of S. saprophyticus, glycol extracts produced significant reductions in biofilms. For five isolates studied, R. officinalis produced greater reductions than CHX. Conclusion: R. officinalis and S. cumini showed effective antibiofilm activity against isolates that showed slime production.

 

Keywords

Biofilm; Rosmarinus officinalis; Slime; Staphylococcus; Syzygium cumini.

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Published

2017-06-28

Issue

Section

Clinical or Laboratorial Research