Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla

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DOI:

https://doi.org/10.4322/bds.2023.e3847

Resumo

Objectives: The present work was designed to evaluate the proliferation and differentiation potential of stem cells from the apical papilla (SCAP) seeded along with platelet rich fibrin (PRF) scaffolds prepared under two different centrifugation protocols. Materials and Methods: Standard and advanced PRF protocols were used. Cells were divided into 4 groups: negative control, positive control, standard (L-PRF) and advanced (A-PRF) groups. Cell count and cell viability assays were carried out to assess the proliferation capacity. Alizarin red S (ARS) stain, Alkaline phosphatase (ALP) activity and Receptor activator of nuclear factor-kappa B ligand (RANKL) immunofluorescence staining were used to evaluate the osteogenic potential in the differentiated cells. Results: Both types of platelet rich fibrin increased the cell count, cell viability with no cytotoxicity that was reflected on increased proliferation and differentiation in terms of the performed tests. Conclusion: A-PRF group showed significant increase in proliferation and differentiation potentials compared to L-PRF group.

KEYWORDS

Alkaline phosphatase; Centrifugation; Platelet rich fibrin; RANKL ligand; Stem cells.

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Publicado

2023-12-20

Como Citar

1.
Shalaby L, Shawkat S, Fathy I. Potential effect of platelet rich fibrin prepared under different centrifugation protocols on stem cells from the apical papilla. BDS [Internet]. 20º de dezembro de 2023 [citado 7º de setembro de 2025];26(4). Disponível em: https://ojs.ict.unesp.br/index.php/cob/article/view/3847

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Seção

Artigos de Pesquisa Clínica ou Laboratorial