The Dynamics of Subcutaneous Tissue Response to Microorganisms Associated with the Extract of Araçá (Psidium cattleianum): An Edemogenic and Microscopic Analysis

Authors

  • Alessandra Cury Machado Department of Biological Sciences – Bauru School of Dentistry – University of São Paulo – Bauru – SP – Brazil.
  • Denise Belucio Ruviere Department of Restorative Dentistry – Araçatuba School of Dentistry – UNESP – Univ. Estadual Paulista – Araçatuba – SP – Brazil.
  • Renata Zoccal Novais Department of Restorative Dentistry – Araçatuba School of Dentistry – UNESP – Univ. Estadual Paulista – Araçatuba – SP – Brazil.
  • Carlos Roberto Emerenciano Bueno Department of Restorative Dentistry – Araçatuba School of Dentistry – UNESP – Univ. Estadual Paulista – Araçatuba – SP – Brazil.
  • Elerson Gaetti Jardim Jr Department of Restorative Dentistry – Araçatuba School of Dentistry – UNESP – Univ. Estadual Paulista – Araçatuba – SP – Brazil.
  • Rodrigo Cardoso De Oliveira Department of Biological Sciences – Bauru School of Dentistry – University of São Paulo – Bauru – SP – Brazil.
  • Eloi Dezan Junior Department of Restorative Dentistry – Araçatuba School of Dentistry – UNESP – Univ. Estadual Paulista – Araçatuba – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2017.v20i2.1408

Abstract

Objective: To evaluate in vivo tissue reaction to the extract of araçá (Psidium cattleianum) associated with inactivated microorganisms. Material and Methods: A 0.1 mL suspension was used containing Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Enterococcus faecalis, Peptostreptococcus micros, and Porphyromonas endodontalis, which were inactivated by heat and mixed into a 1.0 mL saline (control group), an aqueous solution, or a hydroalcoholic extract of araçá. Eighteen male rats (Rattus norvegiccus) under general anesthesia received 0.2 mL of 1% intravenous Evans blue. Thirty minutes later, 0.1 mL of one of the associations was injected into the animals’ dorsal region. The animals were euthanized after 3 and 6 hours, and the materials obtained were placed in formamide for 72 hours then analyzed in a spectrophotometer (?=630 hm). For the morphological analysis, 30 rats received polyethylene tubes implants with the extracts or the saline with the associations in the dorsal region and euthanized after 7 and 30 days to be analyzed according to an inflammation cell score. Results: No significant difference (p>0.05) was observed in the edema among groups. The optical microscopy results showed a repair in the 30-day-period, which was higher when compared to the 7-day-period (p<0.0001). Nevertheless, in the 7-day-period, the hydroalcoholic extract presented a significant response compared to the aqueous extract (p=0.05) and a trend for better results than the control group. Conclusion: The aqueous and hydroalcoholic araçá extracts associated with inactivated microorganisms showed similar responses to control, indicating no interference on the toxic effects of the bacterial components in tissue repair.

Keywords

Anaerobic bacteria; Edema; Inflammation; Plant extracts; Psidium.

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Author Biography

Alessandra Cury Machado, Department of Biological Sciences – Bauru School of Dentistry – University of São Paulo – Bauru – SP – Brazil.

Pos doctor, Department of Biological Sciences

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Additional Files

Published

2017-06-28

How to Cite

1.
Machado AC, Ruviere DB, Novais RZ, Bueno CRE, Jardim Jr EG, De Oliveira RC, et al. The Dynamics of Subcutaneous Tissue Response to Microorganisms Associated with the Extract of Araçá (Psidium cattleianum): An Edemogenic and Microscopic Analysis. BDS [Internet]. 2017 Jun. 28 [cited 2025 Aug. 21];20(2):93-101. Available from: https://ojs.ict.unesp.br/index.php/cob/article/view/1408

Issue

Vol. 20 No. 2 (2017): Apr. - Jun. / 2017 - Published Jun 2017

Section

Clinical or Laboratorial Research

License

Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.

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