Mixing failures of endodontic sealers: an in vivo biocompatibility study

Authors

  • Carlos Roberto Emerenciano Bueno Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil. http://orcid.org/0000-0002-1897-2823
  • Gustavo Arcos Lopes Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Diego Valentim Department of Endodontics – Centro Universitário do Distrito Federal – UDF – School of Dentistry – Brasilia – DF – Brazil.
  • Vanessa Abreu Sanches Marques Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Ana Maria Veiga Vasques Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Marina Tolomei sandoval Cury Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Gustavo Sivieri-Araujo Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Rogerio de Castilho Jacinto Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Luciano Tavares Angelo Cintra Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.
  • Eloi Dezan-Junior Department of Endodontics – Araçatuba School of Dentistry – Univ. Estadual Paulista – UMESP – Araçatuba – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2017.v20i4.1464

Abstract

Objective: Evaluate, in vivo, the influence of mixing failures on endodontic sealers. Material and methods: To alveolus analysis, 80 rats were divided into Sealapex® and AH Plus® groups. Within each group, the sealer was subjected to either partial (incomplete homogenization—simulating handling failures) or total mixing (complete homogenization) over two periods of 7 and 30 days (n = 20). The maxillary incisor was extracted and a polyethylene tube containing the sealer was inserted. To quantify edema, 40 male rats were divided into four groups (n = 10). The animals received 2% Evans Blue intravenously, and either AH Plus® or Sealapex® was injected subcutaneously. The rats were euthanized after 3 or 6 hours and analyzed in a spectrophotometer (630 ?m). To analyze the subcutaneous tissue, 20 rats received polyethylene tube implants with the sealers in the dorsal area (n=10), then euthanized after either 7 or 30 days, and inflammation was evaluated according to an inflammatory cells score. Results: In the alveolar 7-day group, control group presented an inflammation score 1, while all other groups presented a score 2, except AH plus® total mix group (3). After 30 days, all groups presented a score 1. The edemogenic test showed less edema in Sealapex® groups (p < 0.5). In subcutaneous 7-day period, all groups presented score 2. In 30 days, all groups revealed score 1, except AH Plus® partial mix group (2). Conclusion: Regarding mixing of the sealers, there were no significant differences among the groups.

Keywords

AH Plus; Inflammation; Materials testing; Root canal filling materials; Sealapex.

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Published

2017-12-15

Issue

Vol. 20 No. 4 (2017): Oct. - Dec. / 2017 - Published December 2017

Section

Clinical or Laboratorial Research Manuscript