Influence of photobiomodulation and tricalcium silicate-based cement on viability and dentinogenic activity of cultured human dental pulp stem cells

Authors

  • Latifa Mohamed Abdelgawad Cairo University, National Institute of Laser Enhanced Sciences, Medical Laser Applications Department. Giza, Egypt. https://orcid.org/0000-0002-1483-5274
  • Mustafa N.Hasan Cairo University, National Institute of Laser Enhanced Sciences, Medical Laser Applications Department. Giza, Egypt. http://orcid.org/0000-0001-8099-919X
  • Dina Sabry Cairo University, Faculty of Medicine, Medical Biochemistry and Molecular Biology Department. Cairo, Egypt.
  • Marwa Abdelgwad Cairo University, Faculty of Medicine, Medical Biochemistry and Molecular Biology Department. Cairo, Egypt. http://orcid.org/0000-0003-3719-6687

DOI:

https://doi.org/10.4322/bds.2022.e2497

Abstract

Objective: To assess the effect of application of Biodentine (BD), Photobiomodulation (PBM) using 810 nm diode
laser and both on the proliferation and odontogenic differentiation of human dental pulp stem cells (HDPSCs).
Material and Methods: HDPSCs were collected, isolated, and characterized and then divided into six groups:
groups 1, control; groups 2, biodentine (BD); group 3, irradiation at 1 J/cm2 of 810-nm diode laser; group 4,
irradiation at 1 J/cm2 and culture with BD; group 5, irradiation at 2 J/cm2, and group 6, irradiation at 2 J/cm2
and culture with BD. Viability assay was measured through MTT assay and Alkaline phosphatase (ALP) enzyme
activity and mRNA levels of RUNX2, collagen 1 (Col-1) and BMP2 were also assessed. Results: Photobiomodulation
at 1 and 2 J/cm2 combined with biodentine significantly promoted HDPSCs proliferation (in MTT assay results)
and odontogenic differentiation (through the gene expression of RUNX2, Col-1 and BMP2 levels (p < 0.05).
Conclusion: Photobiomodulation at 2 J/cm2 combined with biodentine enhanced proliferation and odontogenic
differentiation of cultured HDPSCs and thus could further be beneficial for dentin regeneration.
KEYWORDS
Human dental pulp stem cells (HDPSCs); PBM; BMP2; RUNX2; COL1.

Downloads

Download data is not yet available.

Author Biography

Latifa Mohamed Abdelgawad, Cairo University, National Institute of Laser Enhanced Sciences, Medical Laser Applications Department. Giza, Egypt.

professor in medical laser applications deparatment ,national instute of laser enhanced siences,cairo university

Downloads

Published

2022-01-18 — Updated on 2022-03-29

Versions

How to Cite

1.
Abdelgawad LM, Hasan M, Sabry D, Abdelgwad M. Influence of photobiomodulation and tricalcium silicate-based cement on viability and dentinogenic activity of cultured human dental pulp stem cells. BDS [Internet]. 2022 Mar. 29 [cited 2025 Aug. 21];25(1). Available from: https://ojs.ict.unesp.br/index.php/cob/article/view/2497

Issue

Vol. 25 No. 1 (2022): Jan - Mar / 2022 Published Jan 2022

Section

Clinical or Laboratorial Research

License

Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.

=================

COPYRIGHT TRANSFER AND RESPONSIBILITY STATEMENT
 (PDF)

For all articles published in the BDS journal, copyright is retained by the authors. Articles are licensed under an open-access Creative Commons CC BY 4.0 license, meaning that anyone may download and read the paper for free. In addition, the article may be reused and quoted, provided that the original published version is cited. These conditions allow for maximum use and exposure of the work while ensuring that the authors receive proper credit. All metadata associated with published articles is released under the Creative Commons CC0 Universal Public Domain Dedication.

Before the submission, authors must obtain permission to reproduce any published material (figures, schemes, tables, or any extract of a text) that does not fall into the public domain or for which they do not hold the copyright. Permission should be requested by the authors from the copyright holder (usually the Publisher, please refer to the imprint of the individual publications to identify the copyright holder).

The authors hereby attest that the study is original and does not present manipulated data, fraud, or plagiarism. All names listed made a significant scientific contribution to the study, are aware of the presented data, and agree with the final version of the manuscript. They assume complete responsibility for the ethical aspects of the study.

This text must be printed and signed by all authors. The scanned version should be submitted as supplemental file during the submission process.