Evaluation of the efficiency of an infection control protocol in dental radiology by means of microbiological analysis

Abstract

The aim of this study was to verify the efficiency of an infection control protocol in dental radiology during radiographic exposure and processing, since the prevention of infectious diseases should be a matter of concern of the professional in all procedures. The areas selected for material collection were the film, tube head, dental chair, lead apron, buttons, protective barrier, darkroom bench and processing solutions (developer solution, water and fixer solution). Material was collected from each area both before and after establishment of the infection control protocol. Ten RODAC plates were employed for each site, adding up to 140 plates (70 before and 70 after), besides 60 Petri plates (30 before and 30 after) containing blood agar as the culture medium. The processing solutions were dropped on the Petri plates, whereas the material collected from the other areas was directly collected on the RODAC plates. All plates were incubated at 37ºC for 48 hours and after this period the number of colonies in each plate was counted. The Wilcoxon test revealed statistically significant differences (p=0.002) between the results before and after establishment of the proposed infection control protocol. It was concluded that the present protocol reduced the number of microorganisms on the analyzed surfaces and processing solutions.