Human umbilical cord vein as a source of osteoblastic cells

Authors

  • Glauce Crivelaro Nascimento Psychobiology Post-Graduate Program – School of Philosophy, Science and Literature of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.
  • Rayana Longo Bighetti Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.
  • Geraldo Aleixo da Silva Passos Júnior Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.
  • Karina Fittipaldi Bombonato-Prado Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2014.v17i3.986

Abstract

Objective: Regenerative medicine and tissue engineering are searching for novel stem cell based therapeutic strategies that will allow for efficient treatment or even potential replacement of damaged organs. The purpose of this work was to study the behaviour of human umbilical cord vein cells (UCVs) through osteoblastic differentiation. Material and Methods: Cells were isolated, expanded and cultivated in osteogenic medium. After 7, 14 and 21 days of culture, there were evaluated cell morphology, proliferation, viability and alkaline phosphatase (ALP) activity. Immunolocalization of ALP was performed  after 1, 7 and 14 days of culture and cells were analysed in a fluorescence microscope. Statistical test utilized was Mann-Whitney (p<0.05). Results: The results showed that osteogenic medium induced morphological changes in the UCVs. Besides, it permited cell viability and proliferation, as well as an increase in the alkaline phosphatase expression and activity. Conclusion: It is concluded that these cells can differentiate into osteoblastic-like cells, contributing to applications for cell therapy and tissue engineering.

Author Biographies

Rayana Longo Bighetti, Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.

Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo

Geraldo Aleixo da Silva Passos Júnior, Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.

Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo (USP)

Karina Fittipaldi Bombonato-Prado, Department of Morphology, Physiology and Basic Pathology – School of Dentistry of Ribeirão Preto – University of São Paulo – Ribeirão Preto – SP – Brazil.

Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo (USP)

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Published

2014-08-18

Issue

Vol. 17 No. 3 (2014): Jul. - Sep. / 2014 - Published August 2014

Section

Clinical or Laboratorial Research