Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

Authors

  • Rafael Avellar de Carvalho Nunes São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.
  • Flávia Lucisano Botelho do Amaral São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.
  • Fabiana Mantovani Gomes França São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.
  • Cecilia Pedroso Turssi São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.
  • Roberta Tarkany Basting São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil. http://orcid.org/0000-0002-5345-5776

DOI:

https://doi.org/10.14295/bds.2017.v20i4.1461

Abstract

Objective: the aim of the present study was to evaluate the influence of adding different concentrations of chitosan to an experimental two-step etch-and-rinse adhesive system on the bond strength and failure mode to dentin. Material and Methods: thirty-two flat dentin surfaces were obtained from extracted human third molars and divided into four groups  (n=8) for application of the adhesive systems: AD - conventional two-step adhesive system (Adper Single Bond 2); EXP – experimental two-step etch-and-rinse adhesive system; Chi0.2% - EXP with addition of 0.2% Chitosan; Chi0.5% - EXP with addition of 0.5% Chitosan. Resin composite build-ups were made and the composite/dentin specimens were sectioned to obtain rectangular beams with a bond area of approximately 1mm2. After 24 hours, the sticks were submitted to microtensile bond strength tests in a universal test machine. The fracture pattern was evaluated under a stereoscopic loupe at 40X magnification. Results: one-way analysis of variance showed that the type of adhesive system had no significant effect on the bond strength values (p = 0.142), showing the mean bond strength values (standard deviation), in MPa, for the groups as follows: AD=20.1 (5.4); EXP=16.6 (2.3); Chi0.2%=16.1 (2.8); Chi0.5%=16.9 (2.3). In all the groups there was predominance of cohesive fractures in dentin, representing 68 to 82% of the failure modes. Conclusion: the addition of 0.2 or 0.5% of chitosan had no influence on the bond strength and failure mode of an experimental two-step etch-and-rinse adhesive system to dentin.

Keywords

Chitosan; Dental Adhesives; Failure Mode; Microtensile Bond Strength.

Author Biography

Roberta Tarkany Basting, São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.

DDS, MSc, PhD, professor, Department of Restorative Dentistry

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Published

2017-12-15

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Section

Clinical or Laboratorial Research Manuscript