Influence of calcium lactate and sodium fluoride solution mouthwashes on enamel microhardness and dentin permeability during in-office bleaching

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DOI:

https://doi.org/10.4322/bds.2022.e3076

Abstract

Objective: This study evaluated the influence of calcium lactate and sodium fluoride mouthwashes on enamel
microhardness and dentin permeability during in-office bleaching. Material and Methods: For the microhardness
evaluation, enamel blocks were randomly submitted to different treatments associated with in-office bleaching
agent using 40% hydrogen peroxide (Opalescence Boost PF 40%/ Ultradent; 3 sessions x 40-minute each)
(n = 10): 1) Control: application of bleaching agent; 2) Calcium lactate: 1-minute immersion before bleaching
treatment; 3) Sodium fluoride: immersion for 1 minute before bleaching treatment; 4) Calcium lactate +
sodium fluoride: 1 minute immersion in calcium lactate solution, followed by 1 minute immersion in sodium
fluoride before bleaching treatment. Dentin discs received the same treatment protocols (n = 10) to evaluate
permeability. Knoop microhardness and dentin permeability assessments were performed twice (at baseline and
48 hours after the end of bleaching treatment). Results: The generalized linear model considering the design of
repeated measures over time showed that there was no significant difference between treatments (p = 0.9577)
and between assessments (p = 0.3267) regarding Knoop microhardness. Kruskal Wallis and Dunn tests showed
that calcium lactate immersion before bleaching treatment provided higher dentin permeability than other
groups (p = 0.0009). Conclusion: The use of sodium fluoride solution and calcium lactate in association with
in-office bleaching treatment did not influence the microhardness of tooth enamel, although calcium lactate
may increase dentin permeability.
KEYWORDS
Tooth bleaching; Dentin; Sodium fluoride; Calcium lactate; Hardness tests.

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Author Biography

Roberta Tarkany Basting, Faculdade São Leopoldo Mandic. Campinas, SP, Brazil.

DDS, MSc, PhD, professor, Department of Restorative Dentistry

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Published

2022-04-13

How to Cite

1.
Gomes L, Trindade M, França F, Amaral F, Basting RT. Influence of calcium lactate and sodium fluoride solution mouthwashes on enamel microhardness and dentin permeability during in-office bleaching. BDS [Internet]. 2022 Apr. 13 [cited 2025 Aug. 21];25(2). Available from: https://ojs.ict.unesp.br/index.php/cob/article/view/3076

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Section

Clinical or Laboratorial Research

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