Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study

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DOI:

https://doi.org/10.4322/bds.2024.e3827

Resumo

Recent scientific evidence suggests a close relationship between estrogen deficiency and vitamin D- related genes. Estrogen and vitamin D were involved with alterations in odontogenesis and tooth eruption process. Objective: The aim of the present study was to evaluate the influence of estrogen deficiency on the expression of genes related to the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model. Material and Methods: This is an experimental clinical study that used female Wistar Hannover rats. The animals were randomly divided into two groups according to the intervention received: Hypoestrogenism Group – animals submitted to estrogen deficiency by ovariectomy surgery and Control Group – animals submitted to sham surgery. Surgical intervention was performed in the prepubertal period; the animals were followed throughout the pubertal period. After euthanasia, the hemimandibles were removed to evaluate the mRNA expression of the vitamin D-related genes AMDHD1, CYP24A1, NADSYN1 and SEC23A in the odontogenic region of incisors through real time PCR. Student’s t test was used to compare means. Kruskal-Wallis test and Dunn’s posttest were also used. The level of significance was 5%. Results: SEC23A was overexpressed in the estrogen deficiency condition in the odontogenic region (p=0.021). Conclusion: Estrogen deficiency may influence the expression of the SEC23A gene involved in the activation and degradation of vitamin D in the odontogenic region of incisors in a murine model.

KEYWORDS

Estrogen; 25-hydroxyvitamin D3; Gene expression; Odontogenesis; Vitamin D.

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Publicado

2024-03-14

Como Citar

1.
do Nascimento CC, Silveira MA, de Paula MC, Taranto MF da R, Schröder A, Kirschneck C, et al. Estrogen deficiency influences SEC23A gene expression in the odontogenic region of incisors – a murine model study. BDS [Internet]. 14º de março de 2024 [citado 23º de outubro de 2025];27(1). Disponível em: https://ojs.ict.unesp.br/index.php/cob/article/view/3827

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Artigos de Pesquisa Clínica ou Laboratorial

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