Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

Authors

  • Fernanda Herrera da Costa Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0002-7885-0692
  • Mateus José Dutra Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0002-8338-3857
  • Luana Marotta Reis de Vasconcellos Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0003-4344-0578
  • Mariana Raquel da Cruz Vegian Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0002-1819-3598
  • Camila Duarte da Silva Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0003-2419-069X
  • Hanna Flávia Santana dos Santos Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0002-5308-5568
  • Claudio Antonio Federico Instituto de Estudos Avançados, Departamento de Ciência e Tecnologia Aeroespacial, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0002-8263-0270
  • Renata Falchete do Prado Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Materiais Dentários e Prótese Dentária, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0001-5970-0464
  • Rubens Nisie Tango Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Materiais Dentários e Prótese Dentária, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0003-0946-1826
  • Estela Kaminagakura Universidade Estadual Paulista, Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, São José dos Campos, São Paulo, Brazil. https://orcid.org/0000-0003-4250-5368

DOI:

https://doi.org/10.4322/bds.2023.e3660

Abstract

Objective: to evaluate the differentiation and gene expression of transcripts related to osteogenesis in a primary
culture of Mesenchymal Stem Cells (MSCs) derived from rat femurs submitted to radiotherapy and the installation
of pure titanium implants. Material and Methods: fifty-four rats received titanium implants in both femurs and
were divided into three groups: Control: implant surgery (C); Implant + immediate irradiation (IrI), and Implant
+ late irradiation (IrL). Euthanasia occurred 3, 14, and 49 days after surgery. The bone marrow MSCs from the
femurs were isolated and cultivated. The cell viability, total protein content, alkaline phosphatase (ALP) activity,
and the formation of mineralization nodules and cellular genotoxicity were analyzed. The gene expression of
Alkaline Phosphatase (phoA), Collagen 1 (COL1), Runt-related transcription factor 2 (RUNX2), Osterix (OSX),
Osteopontin (OPN), Integrin B1 (ITGB1), Bone Sialoprotein (BSP), Osteonectin (SPARC), Osteocalcin (Bglap),
Transforming Growth Factor B-type (TGF-B), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF),
Interleukin-6 (IL-6), Apolipoprotein E (APOE) and Prostaglandin E2 synthase (PGE2) were evaluated by qRTPCR.
Results: ionizing radiation suppresses the gene expression of essential transcripts for bone regeneration,
as well as cellular viability, as observed in the IrI and IrL groups. Conclusion: although this can lead to the loss
of osseointegration and failure of the implant, the MSCs showed more activity at 49 days than at 3 and 14 days.


KEYWORDS
Cancer treatment protocol; Osseointegration; Osteogenesis; Bone regeneration; Dental implants.

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Published

2023-01-11

Issue

Section

Clinical or Laboratorial Research Manuscript