Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats

Autores

  • Daniella Vicensotto Bernardo Department of Bioscience and Oral Diagnosis – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Tabata de Mello Tera Department of Bioscience and Oral Diagnosis – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Andrea Carvalho de Marco Department of Restorative Dentistry – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Antonio Braulino de Melo Filho Department of Social and Pediatric Dentistry – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Mauro Pedrine Santamaria Department of Bioscience and Oral Diagnosis – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil. Department of Restorative Dentistry – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.
  • Maria Aparecida Neves Jardini Department of Social and Pediatric Dentistry – School of Dentistry – Institute of Science and Technology – UNESP – Univ Estadual Paulista – São José dos Campos – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2015.v18i2.1089

Resumo

Objective: This study aimed to quantify through immunohistochemistry the expression of bone formation marker osteocalcin during the repair process of autogenous onlay bone graft, associated or not to the resorbable collagen membrane and to compare these findings with the presence of Diabetes Mellitus. Material and Methods: Sixty rats (Rattus norvegicus, albinus variation, Wistar) aged 90 days, were divided into two groups with 30 animals: test group – rats with induced diabetes; control group - normoglycemic rats. All rats received grafts on the left and right hemi-mandible with or without collagen membrane coverage, respectively. The animals were euthanized at the following periods: 0h, 7, 14, 21, 45, and 60 days. Immunohistochemistry analysis was performed by the marker osteocalcin at receptor site-graft interface. To analyze osteocalcin immunohistochemical expression, a panoramic view photograph of the graft was taken followed by two photographs at larger magnification. Results: No statistically significances at 5% level were observed between diabetic and control group with and without membrane; and diabetic and control groups with membrane coverage. Conclusion: Within the limits of this present study, it can be concluded that the osteocalcin marker might be influenced by diabetes so that it was late expressed during this condition. However, the association of the graft with the membrane could improve this delay by reaching expression values similar to those of control group.

Downloads

Não há dados estatísticos.

Downloads

Arquivos adicionais

Publicado

2015-05-19

Como Citar

1.
Bernardo DV, Tera T de M, de Marco AC, Melo Filho AB de, Santamaria MP, Jardini MAN. Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats. BDS [Internet]. 19º de maio de 2015 [citado 7º de outubro de 2025];18(2):73-81. Disponível em: https://ojs.ict.unesp.br/index.php/cob/article/view/1089

Edição

v. 18 n. 2 (2015): Apr. - Jun. / 2015 - Published May 2015

Seção

Artigos de Pesquisa Clínica ou Laboratorial

Licença

TRANSFERÊNCIA DE DIREITOS AUTORAIS E DECLARAÇÃO DE RESPONSABILIDADE



Toda a propriedade de direitos autorais do artigo "____________________________________________________________________" é transferido do autor(es) para a CIÊNCIA ODONTOLÓGICA BRASILEIRA, no caso do trabalho ser publicado. O artigo não foi publicado em outro lugar e não foi submetido simultaneamente para publicação em outra revista.

Vimos por meio deste, atestar que trabalho é original e não apresenta dados manipulados, fraude ou plágio. Fizemos contribuição científica significativa para o estudo e estamos cientes dos dados apresentados e de acordo com a versão final do artigo. Assumimos total responsabilidade pelos aspectos éticos do estudo.



Este texto deve ser impresso e assinado por todos os autores. A versão digitalizada deverá ser apresentada como arquivo suplementar durante o processo de submissão.


Plaudit

Crossref
Scopus
Google Scholar
Europe PMC

Artigos mais lidos pelo mesmo(s) autor(es)