Influence of different fermentable carbohydrates on dual-species biofilms of S. mutans and A. naeslundii – a pilot study
DOI:
https://doi.org/10.14295/bds.2015.v18i2.1100Abstract
Objective: The aim of this study was to elucidate the effect of presence of different fermentable carbohydrates in the biomass and acidogenicity of biofilm formed by Streptococcus mutans ATCC 25175 in association with Actinomyces naeslundii ATCC 19039. Material and Methods: Single-specie and dual-species biofilms were grown at the bottom of microtiter plates at equal concentration for 24 h at 37 ºC. Carbohydrates were added at 2%: maltose, sucrose, glucose and lactose and as negative control, BHI Broth (0.2% glucose) was used. The pH of each culture was measured to assess acidogenicity after 24 h, immediately after refreshing the culture medium and for the next 30 min, 1 h and 2 h. Crystal violet assay was used as indicator of the total attached biofilm biomass after 24 h incubation and the absorbance was measured at 590 nm. Tukey Multiple Comparison Test was performed for all the statistical analyses. Results: In general, higher amount of biomass was formed by dual-species than single-specie biofilm in the presence of all carbohydrates, except to glucose. In relation to pH, statistically significant differences were observed in S. mutans biofilm only after 24 h, when all carbohydrates showed higher acidogenicity than control group, whereas in dual-species biofilm the highest acidogenicity were found after 24 h for sucrose, lactose, maltose and the control group. Conclusion: The findings indicate that the type of biofilm (single or dual-species) and the carbohydrate used may influence both amount of biomass formed and rate of the pH fall.
Downloads
Downloads
Additional Files
Published
How to Cite
Issue
Section
License
Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.
=================
COPYRIGHT TRANSFER AND RESPONSIBILITY STATEMENT
(PDF)
For all articles published in the BDS journal, copyright is retained by the authors. Articles are licensed under an open-access Creative Commons CC BY 4.0 license, meaning that anyone may download and read the paper for free. In addition, the article may be reused and quoted, provided that the original published version is cited. These conditions allow for maximum use and exposure of the work while ensuring that the authors receive proper credit. All metadata associated with published articles is released under the Creative Commons CC0 Universal Public Domain Dedication.
Before the submission, authors must obtain permission to reproduce any published material (figures, schemes, tables, or any extract of a text) that does not fall into the public domain or for which they do not hold the copyright. Permission should be requested by the authors from the copyright holder (usually the Publisher, please refer to the imprint of the individual publications to identify the copyright holder).
The authors hereby attest that the study is original and does not present manipulated data, fraud, or plagiarism. All names listed made a significant scientific contribution to the study, are aware of the presented data, and agree with the final version of the manuscript. They assume complete responsibility for the ethical aspects of the study.
This text must be printed and signed by all authors. The scanned version should be submitted as supplemental file during the submission process.
