Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis

Authors

  • Erica Crastechini São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Cristiane Yumi Koga-Ito São Paulo State University (Unesp) - Institute of Science and Technology – São José dos Campos – Oral Biopathology Graduate Program – SP- Brazil. São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Environmental Engineering – SP – Brazil.
  • Suzan de Fátima Machado São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Guilherme Rodrigues Teodoro São Paulo State University (Unesp) - Institute of Science and Technology – São José dos Campos – Oral Biopathology Graduate Program – SP- Brazil.
  • Graziella Nuernberg Back-Brito São Paulo State University (Unesp) - Institute of Science and Technology – São José dos Campos – Oral Biopathology Graduate Program – SP- Brazil.
  • Jorgiana Sangalli São Paulo State University (Unesp) - Institute of Science and Technology – São José dos Campos – Oral Biopathology Graduate Program – SP- Brazil.
  • Janete Dias Almeida São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil. São Paulo State University (Unesp) - Institute of Science and Technology – São José dos Campos – Oral Biopathology Graduate Program – SP- Brazil.

DOI:

https://doi.org/10.14295/bds.2018.v21i1.1489

Abstract

Objective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis.

 

Keywords

Ozone; Candida; Antifungal Agents; Stomatitis; Denture.

 

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Published

2018-03-28

Issue

Vol. 21 No. 1 (2018): Jan. - Mar. / 2018 - Published March 2018

Section

Clinical or Laboratorial Research