Alternative therapies for denture stomatitis treatment: in vivo experimental model in rats

Authors

  • Guilherme Rodrigues Teodoro Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Gabriela de Morais Gouvêa Lima Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Marina Bacelar Silva Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Patricia Carla Lopes Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Cybelle Mori Hiraoka Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Gabriela de Fátima Santana-Melo Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista - UNESP
  • Daniel Freitas Alves Pereira Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista- UNESP.
  • Fernanda Lourenção Brighenti Department of Pediatric Dentistry, Araraquara Dentistry School, Universidade Estadual Paulista- UNESP
  • Cristiane Yumi Koga-Ito Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology. Universidade Estadual Paulista- UNESP.

DOI:

https://doi.org/10.14295/bds.2017.v20i1.1368

Abstract

Background: Denture stomatitis (DS) is a multifactorial condition that commonly affects denture users and is mainly caused by Candida albicans. Due to the toxic effects of antifungal therapy, new therapies for DS are claimed. Objective: The aim of the study was to evaluate the efficacy of aqueous extract of Buchenavia tomentosa and sodium bicarbonate against C. albicans in a model of DS in rats. Material and Methods: An acrylic resin device simulating a denture base was fixed covering the palate of forty-eight male rats followed by candidiasis induction. Rats were divided into 4 groups (n=12): Control, sodium bicarbonate, B. tomentosa and nystatin (positive control). Each group was subdivided according to the period of treatment; 24 hours (n=6) and 48 hours (n=6). Animals were sacrificed and had their devices removed for C. albicans counts and SEM analysis. The palate mucosa was removed and processed for histopathologic analysis. Results: After 24 hours of treatment, both B. tomentosa and nystatin groups reduced significantly C. albicans counts when compared to control (nystatin x control, p<0.01; B. tomentosa x control, p=0.03). The results were confirmed by the histologic analysis. Conclusion: Both the aqueous extract of B. tomentosa and sodium bicarbonate was able to significantly decrease C. albicans counts in an experimental model of DS.

Author Biography

Gabriela de Morais Gouvêa Lima, Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, Universidade Estadual Paulista - UNESP

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Published

2017-03-29

Issue

Vol. 20 No. 1 (2017): Jan. - Mar. / 2017 - Published February 2017

Section

Clinical or Laboratorial Research