Scaffold architecture for dental biomaterials: influence of process parameters on the structural morphology of chitosan electrospun fibers

Authors

  • Joyce Rodrigues de Souza São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – SP - Brazil.
  • Tabata Prado Sato São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Dental Materials and Prosthodontics – SP – Brazil.
  • Alexandre Luiz Souto Borges São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Dental Materials and Prosthodontics – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2017.v20i4.1495

Abstract

Objective: The electrospinning is a widely adopted technique used to produce the polymeric fibers. The process depends on process parameters (voltage, flow rate and distance from capillary to the collector). The present study aimed to evaluate the influence of these parameters on chitosan fibers, a biopolymer used as scaffolds in dental and oral tissue engineering. Material and Methods: A solution of 7%(m/w) was prepared dissolving 0.7g of chitosan in 7 mL of trifluoroacetic acid (TFA) and 0.3mL of dichloromethane (DCM) (TFA/DCM - 70:30). After 12 hours, polymer solution (5 mL) was drawn into the syringe and pumped through needles of 0.4 mm internal diameter, at a rate of 0.8 mLh-1, different needle-tip-to-collector distances and voltages, for 10 min.  Electrospun fibers micrographies were obtained using the Scanning Electron Microscope for morphological analysis. Results: All voltages showed significant difference (p < 0.0001) between them. At 15kV fibers showed higher concentration of beads. At 10 and 12 cm of distance there was no statistical significance (p > 0.0001) but at 15 cm beads formation increased significantly (p < 0.0001). At 12 cm fibers showed lowest fibers diameter in comparison to 10 and 15 cm (p < 0.0001). There was no difference between 10 and 12 cm (p > 0.0001) but in comparison to 15 cm both distances presented significantly difference (p < 0.0001).  Conclusion: Thus, it can be concluded that morphology, in chitosan electrospun fibers, is influenced by the voltage and distance and this could describe the mohphological control of these structures.

 

Keywords

Chitosan; Fibers; Electrochemical.

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Published

2017-12-15

Issue

Vol. 20 No. 4 (2017): Oct. - Dec. / 2017 - Published December 2017

Section

Clinical or Laboratorial Research

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All manuscript's copyright ownership of the article "____________________________________________________________________" is transferred from the author(s) to the BRAZILIAN DENTAL SCIENCE, in the event the work is published. The manuscript has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.

We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.

 

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