Determination of chemical elements and cross-section hardness of sclerotic darkened human dentin

Authors

  • Mauricio Yugo Souza São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil. http://orcid.org/0000-0002-0366-5361
  • Laura Celia Fernandes Meirelles São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.
  • Isabela Roberta Vieira Duque São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.
  • Mariane Cintra Mailart São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.
  • Taciana Marco Ferraz Caneppele São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil. http://orcid.org/0000-0003-0521-7922
  • Eduardo Bresciani São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil. http://orcid.org/0000-0003-1665-5733

DOI:

https://doi.org/10.14295/bds.2017.v20i3.1412

Abstract

Objective: The aim of this study was to assess the amount of chemical elements (Ca, O, C, P, Fe, and Mg) and the cross-section hardness of sclerotic darkened dentin in human teeth. Material and Methods: The study was approved by the local IRB and ten extracted teeth (five sound and five presenting sclerotic darkened dentin) were used. Tooth was sectioned mesiodistally and each half was used for each test. Amount of chemical elements (%w) was determined by energy dispersive X-ray spectroscopy (EDS) in three different dentin areas (shallow, medium, or deep sound or sclerotic dentin). Knoop microhardness was determined at the same EDS areas. Data were analyzed by two-way ANOVA and multiple comparison tests, with significance level at 5%. Results: No difference on microhardness was detected between sound and sclerotic dentin (p = 0.743) and also among dentin depths (p = 0.837). Lower Ca (p = 0.024) and higher C (p = 0.015) amounts were found at superficial sclerotic dentin. Increased Mg content (p < 0.001) was detected in sound dentin. Conclusion: It was concluded darkened sclerotic dentin presents similar cross-section microhardness to sound dentin. The assessed chemical elements were similarly present in sound or sclerotic dentin, except for Mg, which was present higher concentration in sound dentin. Ca and P were lower in superficial sclerotic dentin.

 

Keywords: Dentin; Hardness; Minerals; Tooth Remineralization.

Author Biography

Eduardo Bresciani, São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.

 

Departamento De Odonto Restauradora

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Published

2017-09-12

Issue

Vol. 20 No. 3 (2017): Jul. - Sep. / 2017 - Published September 2017

Section

Clinical or Laboratorial Research